Alex Chandler completed 7 team tasks on Inhibiting β-Amyloid Oligomerization

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Alex Chandler completed 5 team tasks on Subcellular Distribution of Puf Proteins

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• Miniprep - isolation of the Tap-tagged Pufs cloned plasmids
• Cloning TAP-tag constructs for 5 Puf family members
• Transformation to Yeast
• Miniprep #2- Isolation of Tap-tagged Puf Plasmids
• Growth rates and cell morphology

Knowledge updates for you have been published recently

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• p38 MAPK Signaling.full.pdf1353073227
  by Daphne Rosenfeld ... 11/16/2012
• Progression of RAS-Mutant Leukemia during RAF Inhibitor Treatment
  by Daphne Rosenfeld ... 11/16/2012
• The Tumor Suppressor CYLD Controls the Function of Murine Regulatory T Cells...
  by Daphne Rosenfeld ... 11/14/2012

J C Hernandez posted 4 figures to the document Puf Family Results

John Folland These look promising!

JC Hernandez We're quite excited by these

Jon Folland added 4 items to the shopping list

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• TaKaRa Ex Taq™ Cat# RR001A from TAKARA
  1 of (250 unit enzyme) for $80.00
• Ethanol Cat# 459844 from Sigma-Aldrich
  1 of (500µl) for $60.00
• Phenol Cat# 185450 from Sigma-Aldrich
  1 of (500g) for $30.00
• Sodium Chloride Cat# S9625 from Sigma-Aldrich
  1 of (1000g) for $60.00

Daniel Moore added 2 experiments to milestone Extract cells, recover mRNA and labelling

• RNA isolation using phenol chloroform
• Extraction of the RBP-mRNA complexes and TEV cleavage